Software for the analysis of scanning fluorescence correlation spectroscopy data

Researchers at Oxford have developed a novel software tool with optimised work-flow for correlating, processing and fitting scanning fluorescence correlation spectroscopy data. It will be used by scientists to process and analyse their scanning correlation data from commercial turn-key microscopes and custom microscopes. At the moment there is no software solution that can do this at bulk levels with as much ease.

Fluorescence correlation techniques are used to investigate photophysical, photochemical, interaction and transport properties of fluorescent or fluorescently labelled molecules at physiological concentrations by analysing the fluctuations of the measured fluorescence signal.

Fluorescence correlation spectroscopy is a powerful quantitative method to study dynamic properties of biophysical systems. It is an emerging technique used in biophysical studies as it exploits the temporal autocorrelation of fluorescence intensity fluctuations originating from a tiny volume.

Fluorescence correlation spectroscopy

Scanning fluorescence correlation spectroscopy is a group of correlation techniques where the measurement volume is moved across the sample in a defined way, resulting in a spatiotemporal correlation of the detected fluorescence.

Scanning improves the accuracy of measurements on slowly moving molecules and allows measurements on systems where other fluorescence correlation approaches perform poorly or are not possible.

The fluorescent solution

The team at Oxford has developed a novel and more efficient method for analysing scanning correlation data from commercial scanning fluorescence scanning microscopy systems. They do this by providing the user with a visualised interface for performing correlation as well as having a high level of automation when compared with existing systems, together with advanced data management and visualisation.

Oxford University Innovation would like to talk to companies interested in licensing this technology.

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