Molecular Biophysics Suite (MBS)
The MBS offers a diverse range of techniques for macromolecule and molecular complex analysis.
The Biophysical Instrumentation Facility and its experienced team of scientists offer a range of techniques for the study and characterization of macromolecules and macromolecular complexes, particularly proteins.
Molecular Biophysics Suite techniques include:
- Size Exclusion Chromatography Multiple Angle Light Scatter (SEC MALS)
- Analytical Ultracentrifugation (AUC)
- Circular Dichroism (CD)
- Dynamic Light Scattering (DLS)
- Fluorescence Spectroscopy (FS)
- Isothermal Titration Calorimetry (ITC)
- Differential Scanning Calorimetry (DSC)
- Surface Plasmon Resonance (SPR)
- Microscale Thermophoresis (MST)
- Stopped Flow
Typical applications include:
- characterisation of macromolecule solution behaviour by SEC MALS and AUC
- protein stability by DSC and CD
- affinity determination by SPR, MST and ITC
Based within the Department of Biochemistry, the facility staff are active users of the equipment in a broad research context and engage with clients to discuss project requirements fully.
Access to the Biophysics Facility can be provided through the facility manager Dr David Staunton: for
- Submission of samples for analysis
- In-house training and hands-on use by client staff
- Consultancy in the application of techniques
Past clients have included biotechnology and global pharmaceutical companies who have accessed a range of techniques to supplement internal resources and expand capabilities.