Single molecule sequencing for single-molecule phenotyping

DNA sequencing is a key method that has had a huge impact on diagnostics, genomics and functional analysis. Although many single-molecule sequencing methods exist, there is currently no proficient way to connect the functional properties of a single DNA molecule with its sequence.

Researchers at the University of Oxford have developed a single-molecule sequencing method capable of connecting the functionality (reactions or interactions) of a single molecule with its sequence. This novel method is expected to support screening libraries of candidate therapeutic or agrichemical targets.

Single-molecule sequencing

Within the last decade, single-molecule sequencing has been utilised in the commercial long-read sequencing market. However, the methods currently used by these companies require specialist equipment and cannot directly link single-molecule phenotype with a DNA sequence. Providing such a link between a single molecule phenotype and its structure would support screening for biomolecules.

There is, therefore, a need for an alternative method that can fulfil this need whilst making use of standard laboratory equipment.

Gap-Seq- a novel sequencing solution

Researchers at the University of Oxford have developed a single-molecule method, called Gap-Seq, that connects the functionality (e.g., chemical reactions and molecular interactions) of large libraries of single nucleic acid molecules (or tagged peptides) with their sequence. This method allows biomolecules of interest (such as aptamers and DNA-binding sites) to be screened rapidly and quantitatively for functionality against specific molecular targets using standard laboratory equipment.


Oxford University Innovation has applied for a patent for this technology and would like to hear from any interested parties who may wish to commercialise this technology.

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