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Single-molecule phenotyping coupled with in situ single-molecule DNA sequencing

Image from Licence Details: Single-molecule phenotyping coupled with in situ single-molecule DNA sequencing

DNA sequencing is a key method that has had a huge impact on diagnostics, genomics and functional analysis. Although many single-molecule sequencing methods exist, there is currently no proficient way to connect the functional properties of a single DNA molecule with its sequence.

Researchers at the University of Oxford have developed a single-molecule sequencing method capable of connecting the functionality (reactions or interactions) of a single molecule with its sequence. This novel method is expected to support screening libraries of candidate therapeutic or agrichemical targets.

Applications: Diagnostics, FRET, SELEX, Aptamer Discovery

Oxford researchers have developed a novel single-molecule sequencing method capable of connecting the functionality of a single nucleic acid molecule with its sequence.

Features Benefits
  • High-Throughput Sequencing: Link the sequence of a single DNA molecule to its functional properties with this advanced high-throughput technique, enabling detailed genetic and phenotypic  analysis.
  • Enables functional sampling of a large sequence space.
  • Offers comprehensive insights into how individual bases affect specific biochemical processes.
  • Identify crucial genetic elements influencing biochemical reactions.
  • Facilitates targeted screening for sequences connected to a phenotype desirable for a certain application.
  • Holistic Measurement Platform: Gain comprehensive insights with one platform that simultaneously measures single-molecule kinetics, protein-DNA interactions, and distances within complexes.
  • Streamlines analysis with integrated measurements.
  • Requires no DNA amplification
  • Enhances data accuracy and consistency, saving time and resources while driving advanced discoveries.
  • Real-Time Interaction Monitoring: Directly detect heterogeneity and monitor interactions and conformational changes in real-time, providing dynamic and accurate data.
  • Provides direct, detailed mechanistic views on dynamic processes.
  • Offers detailed insights into the conformational changes of proteins and/or DNA during protein-DNA interactions.
  • Non-Equilibrium Reaction Analysis: Study non-equilibrium reactions and their kinetics to uncover transient states and detailed mechanisms of DNA-protein interactions.
  • Reveals dynamic processes and transient states of DNA-protein interactions, providing deeper insight into molecular mechanisms that drive biological functions.
  • Understanding the kinetics of DNA-protein interactions aids in designing targeted therapies and more effective treatments for diseases involving these crucial interactions.

Patented & available for:

  • Licensing
  • Co-development
  • Consulting
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