AnaLog-seq: An unbiased method for detecting DNA breakpoints throughout the whole genome
AnaLog-seq is a transformative technology that detects DNA breakpoints, including both single-strand (SS) and double-strand (DS) breaks, across the genome while preserving long-range genetic context.
Applications: Clinical diagnostics; research (e.g. into genome instability, immunity, reproduction, evolution and drug resistance in cancer)
| Features | Benefits |
|---|---|
| Based on long-read sequencing | Precisely localises DNA breakpoints in repetitive and structurally complex genomic regions (where short-read sequencing fails). Quantifies DNA break frequency within individual long DNA molecules. |
| Detects and discriminates between SS and DS breaks | Provides complete information for diagnostic and research purposes |
| Elegantly simple method involving relatively few steps and reagents | Straightforward and inexpensive to apply |
| Current implementation on Oxford Nanopore Technology instrumentation | Widely used sequencing platform |
| Uses synthetic tags that do not occur naturally in biological systems | Simultaneously detects DNA breaks and other epigenetic modifications within the same DNA molecule |
| Relatively small amount of starting material required | Can be scaled up for the analysis of multiple samples |
Patented and Available For
- Co-development
- Consulting
- Licensing