Electrochemical method of sensing target protein molecules

Electrochemical method of sensing target protein molecules.

Applications: Protein detection, protein quantification, protein assaying

Features	Benefits
A method of electrochemical protein quantification involving shotgun redox tagging a protein mixture then specifically recruiting the target protein at an antibody-modified electrode surface	The method is able to discriminate between the target protein and other non-specific proteins with minimal background interference and a high signal-to-noise ratio
The method can be integrated into a microfluidic setup (see image) which could be automated to allow the method to be highly scalable	Integration of a sample protein into the microfluidic system decreased the limit of detection (0.1pg/mL) and required a very small sample (25μg/mL)
Work done on this assay method demonstrates that it can be used for simple and sensitive detection of protein biomarkers	This approach is able to achieve a very low limit of detection while still being applicable across a dynamic range which spans 6 order of magnitude
This assay is generic and can be used to analyse several proteins simultaneously by using an array of electrodes	The method, particularly when integrated into a microfluidic system, is rapid and scalable, leading to a huge reduction in costs and labour requirements